AmScope FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 12MP Color CCD Camera

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2" x 5.6" stage.

The 12MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1" format CCD sensor balances resolution and sensitivity with its 3.1µm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter Set Excitation Filter Dichromatic Mirror Emission Filter Common Stains* UV-1A 330-385nm 400nm 420nm DAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580) V 400-410nm 455nm 455nm B 460-495nm 505nm 510-550nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow G2 510-550nm 570nm 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B *Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications.
Mercury HBO Excitation
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications. Band Peak Wavelength UV 334nm, 365nm V 405nm B 436nm G 546nm Y 578nm

Specifications

Optical System	Infinity-corrected
Head	Trinocular, 30 ° incline, 360 ° rotatable
Interpupillary Adjustment	Compensation-free gemel, 48-75mm
Eyepieces	Plan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port	23mm, compatible with reduction lenses
Nosepiece	Quintuple reversed
Objective Lenses	4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing System	Coaxial coarse and fine focus, upper limit-stop, tension-control
Stage	Double-layer mechanical 185mm x 142mm
X-Y Translation Range	75mm x 55mm
Sub-stage Condenser-holder	Rack-and-pinion, centerable
Condenser	Achromatic swing condenser, NA0.9/0.25
Transmitted Illumination	30W halogen, continuous adjustment
Fluorescence Excitation Illumination	100W mercury-vapor HBO arc-lamp

Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness
4X	Plan achromatic	0.10	28mm	0.17mm
10X	Plan achromatic	0.25	10mm	0.17mm
20X	Plan achromatic	0.40	5.1mm	0.17mm
40X	Plan achromatic	0.65	0.7mm	0.17mm
100X	Plan achromatic	1.25	0.18mm	0.17mm

Camera Specifications

Packing List:

• One trinocular head
• One microscope body
• One photo port assembly
• Five objective lenses: 4X, 10X, 20X, 40X, 100X
• One pair 10X eyepieces
• One stage assembly
• One swing condenser
• One halogen illuminator assembly
• One episcopic illuminator
• One episcopic lamp housing
• One power supply for episcopic illuminator
• One eye shield
• Two AC power cords (US and Canada standard)
• One hex driver
• One dust cover
• One sample immersion oil
• One USB 3.0 CCD Fluorescence Digital Camera
• One USB 3.0 Cable
• One C-Mount